Dino

Collagenase Type II

Collagenase Type II

Synonyms Clostridiopeptidase A

CAS Number: 2593923

EC Number: 232-582-9 MDL Number: MFCD00130830

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SKU 02100502-CF
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Description

Product Description

Collagenase Type Ii

Application Notes

Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Release of cells from animal tissues

Specifications
SKU 02100502-CF
Application Notes Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.
EC Number 232-582-9
Format lyophilized powder
Hazard Statements H315-H319-H334-H335
Applications Release of cells from animal tissues
Pack Size No
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Brown Lyophilized Powder
Product Families Description Collagenase
Product Overview Collagenase Type Ii
Safety Symbol GHS07, GHS08
Source Clostridium histolyticum
Specific Activity >125 u/mg solid
Specificity Clostridial collagenase I or clostridiopeptidase A degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence -Pro-Y-Gly-Pro- where Y is most frequently a neutral amino acid. This bond in synthetic peptide substrates may also be split.
Storage and Handling Store at +4 °C. Store Desiccated.
Unit Definition One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.