Dino

Protein A

Protein A

Synonyms Protein A from Staphylococcus aureus

CAS Number: Not applicable

MDL Number: MFCD00081934

Packaging size: 20 mg

SKU 0215005020
Price:
$528.10
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Description

Product Description

Protein A

Application Notes

Protein A may be conjugated with various reporter molecules including fluorescent dyes (FITC), enzyme markers (peroxidase, β-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including western blotting, immunohistochemistry, and ELISA applications. In addition, protein A may be immobilized on a solid support such as agarose or acrylic beads for the purification of either polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Detection of immunoglobulins in various immunochemical assays||Purification of either polyclonal or monoclonal immunoglobulins

Specifications
SKU 0215005020
Application Notes Protein A may be conjugated with various reporter molecules including fluorescent dyes (FITC), enzyme markers (peroxidase, β-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including western blotting, immunohistochemistry, and ELISA applications. In addition, protein A may be immobilized on a solid support such as agarose or acrylic beads for the purification of either polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays.
Biochemical Physiological Actions Protein A is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel α-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat. Protein A also participates in a number of different protective biological functions including anti-tumor, toxic, and carcinogenic activities. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.
Capacity Binding Capacity: 6-8 mg of human IgG per mg solid(Lit.)
Extinction Coefficient E1%280 = 1.4 (Lit.)
Format Frozen Solid
Gene ID Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448)
Isoelectric Point 4.85 - 5.10(Lit.)
Applications Detection of immunoglobulins in various immunochemical assays||Purification of either polyclonal or monoclonal immunoglobulins
Pack Size 20 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Frozen Solid
Product Families Description Protein A
Product Overview Protein A
Source Staphylococcus aureus
Specificity Protein A will bind the Fc portion of human IgG subclasses; IgM; IgA and IgE; and mouse IgG1 (weakly); IgG2a and IgG2b. Protein A also binds IgGs from other laboratory and domestic animals; including monkey; rabbit; pig; guinea pig; dog and cat.
Storage and Handling Store at -20 °C. Store Desiccated.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.