Dino

5-Bromo-4-Chloro-3-Indolyl-Β-D-Galactopyranoside

5-Bromo-4-Chloro-3-Indolyl-Β-D-Galactopyranoside

Synonyms X-Gal; 5-Bromo-4-chloro-3-indolyl-β-D -galactoside; BCIG

CAS Number: 7240-90-6 Molecular Formula: C14H15BrClNO6 Molecular Weight: 408.629 g/mol

Beilstein Registry Number: 1402009 EC Number: 230-640-8 MDL Number: MFCD00005666

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SKU 02194811-CF
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Description

Product Description

5-Bromo-4-Chloro-3-Indolyl-Β-D-Galactopyranoside

Application Notes

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Indigogenic substrate for β-galactosidase

Specifications
SKU 02194811-CF
Application Notes 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.
Beilstein Registry Number 1402009
EC Number 230-640-8
Format powder
Grade Molecular biology reagent
Applications Indigogenic substrate for β-galactosidase
Molecular Formula C14H15BrClNO6
Molecular Weight 408.629 g/mol
Optical Rotation (c=1, DMF:H2O 1:1): -62.0 ° (Lit.)
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Powder
Product Families Description 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
Product Overview 5-Bromo-4-Chloro-3-Indolyl-Β-D-Galactopyranoside
Purity ≥98%
Storage and Handling Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.