Dino

Tris-Glycine Buffer

Tris-Glycine Buffer

CAS Number: Not applicable

Packaging size: 1 box

SKU 04816200
Price:
$82.65
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Description

Product Description

Tris-Glycine Buffer

Application Notes

Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Used for Western blotting and gel electrophoresis

Specifications
SKU 04816200
Application Notes Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
Components 0.025 M Tris, 0.192 M Glycine
Format powder
Applications Used for Western blotting and gel electrophoresis
Pack Size 1 box
Personal Protective Equipment Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Physical Appearance Fine White Powder
Preparation Method Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use.
Product Families Description Tris-Glycine Buffer
Product Overview Tris-Glycine Buffer
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.