Mycoplasma Hoechst Stain Kit
Mycoplasma Hoechst Stain Kit is designed for rapid in situ detection of mycoplasma, and other prokaryotic organisms, in cell cultures in less than two hours. This procedure is based on the Hoechst Stain method cited by the Tissue Culture Association (TCA Procedure No, 75361.) Several DNA fluorochromes such as DAPI, quinacrine mustard and quinacrine dihydrochloride have been used in the same technique but none of the fluorochromes perform as well as the Hoechst stain with respect to fluorescent effect, slow quenching and minimum background fluorescence. The Hoechst Stain method selectively binds minor grooves of the DNA, which allows for efficient staining of the nuclei.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Detection of mycoplasma
|Applications||Detection of mycoplasma|
|Materials Required Not Supplied||Sterile distilled water for dilution of 10X HBSS, Incubator (37 °C), refrigerator, various glassware, fluorescent microscope, Carnoy's fixative or 1 part glacial acetic acid to 3 parts absolute methanol.|
|Personal Protective Equipment||Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Handle with gloves skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Kit with 4 Components|
|Research Areas||Immunostaining and Detection||Immunostaining and Detection||Microbiological Analysis||Contamination, Prevention, Detection & Removal|
|Specificity||This stain is highly specific for DNA. It is thought to occupy one of the grooves of the DNA double helix; binding by intercalation. The stain will cause cellular nuclei to fluoresce; however; the other major DNA cytoplasmic components - mitochondria - will not. All prokaryotic organisms (Mycoplasmas; bacteria; yeast; fungi) will give off fluorescence. The identification of the contaminant must be made by noting size; morphology and relationship to the cell.|
|Storage and Handling||Concentrated Hoechst stain, Hanks Balanced Salt Solution, slides and mounting medium may be stored at 2 C to 8 °C or below; Store kit at +4 °C.|
|Typical Working Concentration||The Hoechst Stain should give maximum fluorescent intensity when diluted 1:10 with Hanks Balanced Salt Solution, pH 7.0, achieving a final concentration at 0.05 µg/mL.|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|