TRANSFORM & GROW BACTERIAL TRA
- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time
EZ-YEAST TRANSFORMATION KIT
- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA
CIRCLEGROW (STERILE SOLUTION)
Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.
ALKALI-CATION YEAST TRANSFORMA
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose
RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.
RPMI 1640 Without L-Glutamine
RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.
Mp-Mrc-5 Serum-Free Cell Culture Medium
MP-MRC-5® Medium, Serum Free, very low protein (90 ng/mL) medium developed specifically for the culture of MRC-5 cells. MP-MRC-5™ medium was developed specifically for microcarrier culture of MRC-5 cells in production systems. It is completely animal component-free and contains very little protein. MP-MRC-5™ achieves growth rates superior to standard serum-supplemented media. Adaptation from serum-supplemented media is not necessary.
TCH® Defined Serum Replacement, 50× Concentrate
TCH is a fortified, multipurpose serum replacement for long-term culturing of primarily human anchorage-dependent and suspension cells. TCH® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.
TCM® Defined Serum Replacement
TCM™ is a fortified, multipurpose serum replacement for long-term culturing of many types of anchorage dependent and suspension cultures. TCM® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.
100 mM Sodium Pyruvate (11.00 mg/mL)
Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.
Hat Supplement (50X Solution)
(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.
ITS Premix Solution
ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.
Supplement VX is recommended as an enrichment for the cultivation of Neisseria gonorrhoeae, Haemophilus influenzae and other fastidious organisms. Supplement VX is a sterile lyophilized concentrate of essential growth factors for supplementing media for culturing microorganisms with exacting growth requirements. It is used for the preparation of chocolate agar from GC Agar Base and hemoglobin for isolation and cultivation of N. gonorrhoeae. It is also recommended for enriching Brain Heart Infustion Agar and Brain Heart Infusion Broth