Culture Media, Powder
Columbia Agar Base
Columbia Agar Base is a highly nutritive general purpose medium for the cultivation of fastidious organisms, especially when supplemented with plain or chocolated blood. Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.
Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate
Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate
Terrific Broth, Modified
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Tryptic Soy Agar
Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.
Tryptic Soy Broth
Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.
Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).