Culture Media, Powder
Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine without Sodium Carbonate
One of the most commonly used of all cell culture media for a variety of cell lines.
Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate
Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate
High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate
High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.
Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate
Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.
Malt Extract Agar (MEA)
Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks. It is also recommended in conjunction with other specific media which are included in this manual. A medium for the isolation and enumeration of yeasts and molds. It can be used in cultivation of the strains for microbiological vitamin assays.
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Potato Dextrose Broth
Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can be used in the analysis of dairy products, bottled drinks, frozen food and other types of food. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides. When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45-50 °C, approximately 14 mL of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.