Secondary Antibodies, Rabbit
Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification
Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.
Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.
MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:
- High specificity for primary antibody species and isotypes
- Multiple pre-conjugations for sensitive detection
- Various fragments
- High purity
Rabbit Anti-Sheep IgG (Fc Fragment) Affinity Purified, Alkaline Phosphatase Conjugated
Form: affinity purified, lyophilized
Applications: immunoblotting and ELISA.
Alkaline Phosphatase Conjugated
Rabbit Antiserum To Bovine IgG (Whole Molecule)
Product is the lyophilized powder of rabbit antiserum to bovine IgG (whole molecule) and buffer salts. The antiserum is prepared by hyperimmunizing rabbits with highly purified bovine IgG; antiserum is delipidated and made specific by solid-phase absorption.
Rabbit IgG Fraction To Bovine IgG (Whole Molecule)
Product is the lyophilized powder of rabbit IgG fraction to bovine IgG (whole molecule) and buffer salts
Rabbit, Anti-Goat IgG Fc Fragment, Affinity Purified
Form: affinity purified liquid
Applications: ELISA, IEP and gel diffusion
Rabbit IgG Fraction To Hamster IgG (Whole Molecule)
Anti-HamsterHost: rabbitForm: lyophilized (IgG)Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Rabbit IgG Fraction To Human IgG (Whole Molecule)
Product is the lyophilized powder of rabbit IgG fraction to human IgG (whole molecule) and buffer salts. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Rabbit Affinity Purified Antibody To Mouse IgG (Whole Molecule)
Product is rabbit affinity purified antibody to mouse IgG (whole molecule) and buffer salts.
Fluorescein-Conjugated Rabbit IgG Fraction To Chicken IgG (Whole Molecule)
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to chicken IgG (whole molecule) and buffer salts.
Alkaline Phosphatase-Conjugated Rabbit Affinity Purified Antibody To Goat IgG (Whole Molecule)
Product is alkaline phosphatase (AKP)-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts.
Peroxidase-Conjugated Rabbit IgG Fraction To Swine IgG (Whole Molecule)
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit F(ab')2 fragment to swine IgG (whole molecule) and buffer salts.